Before you can transfer and store mushroom cultures on agar you need properly made nutrient rich agar plates.
Culture types of agar plates.
An agar plate an example of a bacterial growth medium.
Starting with sterilized agar plates.
A growth medium or culture medium is a solid liquid or semi solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation 1 or.
Store nutrient agar plates in refrigerator.
You can buy nutrient agar powder and sterile petri dishes and prepare nutrient agar plates by yourself.
Agar plates come with many different types of media or nutrients depending on the microorganism you want to culture.
Microbial growth media contains nutrients and an energy source to fuel the microbes as they grow and agar to keep the media in a semi solid gel like state.
On solid media a single microbe will grow and divide to produce a colony a spot of identical descendants.
We have premade or called pre prepared nutrient agar plates made with sterile petri dishes at very affordable.
Blood agar plates blood agar plates bap are made by adding five to ten percent sheep or horse blood to the nutrient medium.
The orange lines and dots are formed by bacterial colonies.
Blood agar plates baps contain mammalian blood usually sheep or horse typically at a concentration of 5 10.
Baps are enriched differential media used to isolate fastidious organisms and detect hemolytic activity.
Agar plates are the standard solid support material for growing microorganisms.
Specifically it is a streak plate.
β hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
But it takes time and kind of messy and sometimes you can screw up your project.
The basic process for doing this is to mix up a nutrient rich agar solution pressure sterilize it and then pour the mix onto sterile dishes in a clean environment.
In the above picture of a mixed culture an agar plate that has been exposed to the air and many different colony morphologies can be identified.